Journal: Experimental Neurobiology

Article Title: The Effect of Donor-Dependent Administration of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells following Focal Cerebral Ischemia in Rats

doi: 10.5607/en.2015.24.4.358

Figure Lengend Snippet: (A) Rotarod test. (B) Limb placement test. G19, G16, G5 hUCB-MSC-treated rats showed an improved functional behavioral outcome compared to the control group. (C) Infarct size. Reduced infarct size in MCAO rats that received hUCB-MSCs. (D) Representative cresyl violet staining images revealed that the ischemic site in rats that received hUCB-MSCs after MCAO was significantly smaller than that in rats in the PBS group. Scale bar=1000 µm. Data are expressed as the mean percentage of the infarct size±SEM ( * p<0.05 experimental vs. control group) relative to the size of the intact hemisphere.

Article Snippet: The rotarod test (Panlab, Barcelona, spain) consisted of an accelerating rod, which provided an index of forelimb and hindlimb motor coordination and balance [ ].

Techniques: Functional Assay, Control, Staining

Journal: EMBO Molecular Medicine

Article Title: A combination of two human monoclonal antibodies cures symptomatic rabies

doi: 10.15252/emmm.202012628

Figure Lengend Snippet: A Rabies virus infection kinetics in the central nervous system of infected mice. Tha‐RABV load (copies/μg of RNA) detected in the thoracolumbar spinal cord, in the brainstem plus cerebellum, and in the cerebral cortex of infected mice according to different time points post‐infection ( n = 4 per time point). nd: not detected. Horizontal lines indicate the median. B Follow‐up of the clinical signs of mice under different treatments. Heat maps were established based on a progressive 0–7 clinical score scale (0: no apparent changes; 1: ruffled fur; 2: slow movement, hind limb ataxia; 3: apathy; 4: monoplegia; 5: hind limb paralysis, tremors; 6: paralysis, conjunctivitis/keratitis, urine staining of the haircoat of the perineum; 7: death). Each line represents one animal throughout time. C Body weight progression of mice under different treatments. Mice were weighed on a daily basis during the treatment administration and then twice a week up to 100 days post‐infection. All the mice were equipped with iPRECIO pumps weighing ca. 3.3 g. Each line represents one animal throughout time. D, E Mouse behavioral testing. The tested animals were non‐infected ( n = 3 mice with iPRECIO pump + n = 4 age‐related mice without iPRECIO pump), infected non‐treated ( n = 5), infected and treated at 7 dpi ( n = 5, mice #9 to #13), and infected and treated at 8 dpi ( n = 5, mice #24 to #28). (D) Open‐field test performed at 7 dpi (prodromal phase) and after the remission of clinical signs for the survivors (80 dpi). (E) Rotarod motor performance at 7 dpi (prodromal phase), 8 dpi (symptomatic phase), and after the remission of clinical signs for the survivors (20, 30, and 60 dpi). Horizontal lines indicate the median. Kruskal–Wallis followed by Dunn’ multiple comparisons test (7 and 8 days post‐infection) and Mann–Whitney test (20, 30, and 60 days post‐infection). The rotarod performance presented a negative correlation (Spearman's r = −0.768) with the clinical score at 8 dpi. * P < 0.05, ** P < 0.01, and *** P < 0.001. Treatment description = NI: non‐infected ( n = 5); NT: infected, non‐treated ( n = 5); infected, treated at 6 dpi (2 + 2 mg/kg) ( n = 4); infected, treated at 7 dpi (2 + 2 mg/kg) ( n = 9); infected, treated at 8 dpi (2 + 2 mg/kg) ( n = 15); infected, treated at 8 dpi (10 + 10 mg/kg) ( n = 4). Exact P values are shown in Appendix Table S2 .

Article Snippet: Mice were placed on an accelerating rotarod (Letica LE8200, Bioseb) at 7 dpi (prodromal phase), 8 dpi (symptomatic phase), and after the remission of clinical signs for the surviving ones (20, 30, and 60 dpi).

Techniques: Virus, Infection, Staining, MANN-WHITNEY