Journal: Scientific Reports

Article Title: Novel roles of phentolamine in protecting axon myelination, muscle atrophy, and functional recovery following nerve injury

doi: 10.1038/s41598-022-07253-w

Figure Lengend Snippet: Assessment of post-injury functional recovery and sciatic nerve axon morphology. ( A ) Mice body weights in grams ( n = 8–12 per treatment group per time point). ( B ) Rotarod test was performed to access the motor coordination and balance of the mice before, 7 and 14-day post-injury (dpi) in mice treated with saline and phentolamine. The latency until the mice fell off the rotating rod was monitored for 300 s ( n = 8–12 per group at each time point). Two-way ANOVA with Benjamini and Hochberg false discovery rate correction for multiple comparisons was performed to determine the significance. ( C ) Sciatic function index (SFI) was evaluated by performing an inked-foot print test at pre-injury, 7, and 14 dpi in both treatment groups ( n = 8–12 per group per time point). Two-way ANOVA with Benjamini and Hochberg false discovery rate correction for multiple comparisons was performed to determine the significance. ( D – F ) Transverse semithin sections of 14 dpi sciatic nerves stained with toluidine blue from control, saline, and phentolamine treated mice (scale bar, 50 µm). ( G ) Myelin sheath thickness was measured as previously done . One-way ANOVA with Benjamini and Hochberg false discovery rate correction for multiple comparisons was performed to determine the significance. ( H ) Area based g-ratios were measured using an image J plugin, g-ratio calculator by analyzing of a total of 150 randomly chosen myelinated axons (excluding the axons whose myelin was touching another neighboring axons) from the sections of individual sciatic nerves per treatment group (control n = 3, saline n = 5, phentolamine n = 4). Kruskal–Wallis test (non-normally distributed data) followed by Benjamini and Hochberg false discovery rate correction for multiple comparisons was performed. ( I ) Quantification of the mean axon count in 200 µm 2 area selected from the middle of the sciatic nerve section image (control n = 3, saline n = 5, phentolamine n = 5). One-way ANOVA followed by Benjamini and Hochberg correction was performed to calculate statistical significance. Data are shown as Mean ± SEM (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).

Article Snippet: Mice were assessed for sensorimotor coordination before-and-after sciatic nerve injury utilizing the Rotarod apparatus (Stoelting, UgoBasile) consists of a rotating rod of at the height of 20 cm from the floor.

Techniques: Functional Assay, Saline, Staining, Control

Journal: PLoS ONE

Article Title: Loss of p53 Ser18 and Atm Results in Embryonic Lethality without Cooperation in Tumorigenesis

doi: 10.1371/journal.pone.0024813

Figure Lengend Snippet: Performance of wildtype (WT) and p53 S18A/S18A animals on a rotarod apparatus. Data for female mice is on the left and male mice on the right. A–B Weight of animals tested. Data are presented as mean ± standard deviation. C–D Training of animals on rotarod. 4–5 month old animals were trained for 10 trials separated by 5 min. on an accelerating rotarod (0–40 rpm). The latency to fall was recorded (n = 5–6 mice/genotype/gender). E–F Average of three experimental trials 24 hrs. after training. Data are presented as mean ± standard deviation. * P <0.05, ** P <0.01. Data were analyzed by two-tailed student's t-test (A, B) and One-Way ANOVA followed by Tukey post-hoc (C–F).

Article Snippet: The rotarod apparatus (Stoelting) was used to measure motor coordination and balance, as well as the ability of mice to improve motor skill performance with training.

Techniques: Standard Deviation, Two Tailed Test